Human cryptosporidiosis: detection of specific antibodies in the serum by an indirect immunofluorescence
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چکیده
منابع مشابه
Detection of Cryptosporidium oocysts in human fecal specimens by an indirect immunofluorescence assay with monoclonal antibodies.
An indirect fluorescence assay (IFA) with monoclonal antibodies developed for the detection of Cryptosporidium oocysts in fecal smears (Meridian Diagnostics, Inc., Cincinnati, Ohio) was compared with the Zeihl-Neelsen-modified acid-fast stain (MAFS) in 119 human fecal specimens collected between 1984 and 1987. The sensitivity of the IFA was 100%; all 56 specimens positive by MAFS exhibited fluo...
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BACKGROUND HEp-2 cells that overexpress the human 60-kDa SSA antigen have been used to improve sensitivity and specificity for the detection of anti-SSA antibodies by indirect immunofluorescence. We describe a survey on the detection of anti-SSA antibodies using a commercial substrate that overexpresses SSA. METHODS The evaluation was done on 18 371 consecutive samples submitted to the labora...
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An indirect immunofluorescence test was both sensitive and specific in detecting IgG antibodies to Aspergillus fumigatus. The test revealed a significant increase in titre of antibodies in patients with the allergic or mycetomal forms of pulmonary aspergillosis.
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The detection of immunoglobulin M (IgM) antibodies to cytomegalovirus-induced late antigens by an indirect immunofluorescence assay was improved by the use of monoclonal antibodies to human IgM. Nonspecific background fluorescence was absent, facilitating the reading of the slides and the detection of a specific fluorescence reaction in sera with low levels of specific IgM. Moreover, the indire...
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Hepatitis A virus-specific BSC-1 cells were used for the detection of serum immunoglobulins to hepatitis A virus by indirect immunofluorescence. Of 150 serum samples tested, specific immunoglobulin M was detected only in patients with serologically confirmed acute hepatitis A, while specific immunoglobulin G was detected in patients with acute or past clinical hepatitis A as well as many patien...
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ژورنال
عنوان ژورنال: Revista de Saúde Pública
سال: 1996
ISSN: 0034-8910
DOI: 10.1590/s0034-89101996000500001